2GLS
REFINED ATOMIC MODEL OF GLUTAMINE SYNTHETASE AT 3.5 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.500, 134.500, 200.100 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 3.500 |
| R-factor | 0.258 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.040 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.500 * |
| Number of reflections | 65223 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.1 * | 21 * | took from Jonson et al.,(1984) from original paper * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | imidazole | 7 (mM) | |
| 2 | 1 | drop | 1.1 (mM) | ||
| 3 | 1 | drop | MPD | 1 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 1 (mM) | |
| 5 | 1 | reservoir | MPD | 5 (%) |
