2GLR

MOLECULAR STRUCTURE AT 1.8 ANGSTROMS OF MOUSE LIVER CLASS PI GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-(P-NITROBENZYL)GLUTATHIONE AND OTHER INHIBITORS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	101.950, 77.640, 57.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.169
Rwork	0.169
RMSD bond length	0.010
RMSD bond angle	2.563
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	2.200 *	2.000 *
Rmerge	0.037 *
Number of reflections	22084 *
Completeness [%]	91.0 *	85 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	15 (mg/ml)
2	1	drop	MES	10 (mM)
3	1	drop	glutathione sulphonic acid	18 (mM)
4	1	drop	MES	50 (mM)
5	1	drop		0.02 (%)
6	1	drop	PEG6000	20 (%)
7	1	drop	imidazole	50 (mM)
8	1	reservoir	sodium/phosphate phosphate	0.3 (M)