2GLK
High-resolution study of D-Xylose isomerase, 0.94A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.712, 97.935, 101.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.360 - 0.940 |
| R-factor | 0.1157 |
| Rwork | 0.115 |
| R-free | 0.12792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xib |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.917 |
| Data reduction software | X-GEN |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.360 | 0.965 |
| High resolution limit [Å] | 0.940 | 0.944 |
| Number of reflections | 252079 | |
| Completeness [%] | 85.6 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 50mM tris-HCl,25%AMSO4,2mM Mn2+ and 2mM Co2+ , XI @ 25mg/ml., pH 8, VAPOR DIFFUSION, temperature 293K |
