2GL9
Crystal Structure of Glycosylasparaginase-Substrate Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.118, 96.745, 61.998 |
| Unit cell angles | 90.00, 90.58, 90.00 |
Refinement procedure
| Resolution | 17.350 - 2.000 |
| R-factor | 0.174 |
| Rwork | 0.170 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gac |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.350 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.076 | 0.337 |
| Number of reflections | 39436 | |
| <I/σ(I)> | 14.1 | 2.8 |
| Completeness [%] | 99.2 | 94.7 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15% PEG 3350, 100 mM HEPES, 0.1% sodium azide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
