2GL3
Crystal structure of Mycobacterium tuberculosis trHbN, TyrB10Phe GlnE11Val mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-29 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.855, 61.684, 90.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.920 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 18928 | |
| Completeness [%] | 95.2 | 76.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | sodium dihydrogen phosphate + dipotassium hydrogen phosphate 1.8 M, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
