2GL0
Structure of PAE2307 in complex with adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.862, 109.825, 112.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 2.250 |
| Rwork | 0.170 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wvq |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.297 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.509 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.071 | 0.238 |
| Total number of observations | 19415 | |
| Number of reflections | 45062 | 6169 |
| <I/σ(I)> | 7.8 | 3 |
| Completeness [%] | 98.4 | 94.6 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 291 | 21% (w/v) MPEG 5000, 0.2M AMPSO/KOH, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
