2GKO
S41 Psychrophilic Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-01 |
| Detector | BRUKER |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.000, 61.000, 174.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.700 - 1.400 |
| R-factor | 0.1603 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yja |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.360 |
| Data scaling software | SAINT (V5.I07) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 1.460 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 43299 | |
| <I/σ(I)> | 1555.4 | |
| Completeness [%] | 83.6 | 66.25 |
| Redundancy | 4.38 | 4.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20-35% (w/v) PEG-8000 100mM HEPES (pH 7.0-7.2) 2ul protein @ 8.0mg/ml + 2ul reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
