2GKO
S41 Psychrophilic Protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-03-01 |
Detector | BRUKER |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.000, 61.000, 174.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.700 - 1.400 |
R-factor | 0.1603 |
R-free | 0.19770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yja |
RMSD bond length | 0.011 |
RMSD bond angle | 1.360 |
Data scaling software | SAINT (V5.I07) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.700 | 1.460 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 43299 | |
<I/σ(I)> | 1555.4 | |
Completeness [%] | 83.6 | 66.25 |
Redundancy | 4.38 | 4.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20-35% (w/v) PEG-8000 100mM HEPES (pH 7.0-7.2) 2ul protein @ 8.0mg/ml + 2ul reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 277K |