2GKG
Receiver domain from Myxococcus xanthus social motility protein FrzS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 63 |
| Unit cell lengths | 64.360, 64.360, 46.038 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-factor | 0.0916 |
| Rwork | 0.092 |
| R-free | 0.12350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GUANYLATE CYCLASE RECEIVER DOMAIN (PDB CODE: 1W25) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.061 | 0.492 |
| Number of reflections | 58567 | |
| <I/σ(I)> | 28.75 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, NaFormate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
