2GK9
Human Phosphatidylinositol-4-phosphate 5-kinase, type II, gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.953 |
Spacegroup name | P 43 |
Unit cell lengths | 95.390, 95.390, 189.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.261 |
Rwork | 0.254 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.663 | |
Number of reflections | 40823 | |
<I/σ(I)> | 8.23 | 2.13 |
Completeness [%] | 98.3 | 99.7 |
Redundancy | 2.55 | 3.18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 20 % Peg3350 0.3 M di-ammoniumtartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |