2GJW
RNA Recognition and Cleavage by an Splicing Endonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.965, 140.413, 82.048 |
Unit cell angles | 90.00, 111.68, 90.00 |
Refinement procedure
Resolution | 35.090 - 2.850 |
R-factor | 0.24986 |
Rwork | 0.247 |
R-free | 0.29646 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.831 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.090 | 2.920 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 40048 | |
Completeness [%] | 90.2 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | VAPOR DIFFUSION, HANGING DROP, PH 6.5, 303K, 0.12M AMMONIUM ACETATE, 35mM MAGNESIUM ACETATE, 50mM SODIUM CACODYLATE,10% ISOPROPANOL |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | AMMONIUM ACETATE | ||
2 | 1 | 1 | MAGNESIUM ACETATE | ||
3 | 1 | 1 | SODIUM CACODYLATE | ||
4 | 1 | 1 | ISOPROPANOL | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | AMMONIUM ACETATE | ||
7 | 1 | 2 | MAGNESIUM ACETATE | ||
8 | 1 | 2 | SODIUM CACODYLATE |