2GJV
Crystal Structure of a Protein of Unknown Function from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00882 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.137, 76.995, 85.112 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 27.900 - 2.390 |
| R-factor | 0.17194 |
| Rwork | 0.168 |
| R-free | 0.24883 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.576 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.071 | 0.116 |
| Number of reflections | 30366 | |
| <I/σ(I)> | 16.3 | 9.7 |
| Completeness [%] | 94.0 | 93.8 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | 0.2 M Calcium Chloride dihydrate, 0.25 Sodium Chloride, 0.01 M TRIS-HCL, 20%w/v PEG3350, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
