2GJU
Crystal structure of hypothetical protein PH1004 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.742, 129.571, 113.557 |
| Unit cell angles | 90.00, 104.46, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.281 |
| Number of reflections | 91840 | |
| <I/σ(I)> | 8.5 | 3.6 |
| Completeness [%] | 98.3 | 95.7 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | 0.05M Potassium dihydrogen phosphate, 10% PEG 8000, MICROBATCH, temperature 291K |
