2GJS
The crystal structure of human RRAD in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.427, 58.560, 53.603 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 39.400 - 1.900 |
| R-factor | 0.19726 |
| Rwork | 0.195 |
| R-free | 0.23538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | swissmodel (first approach mode) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.605 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 24816 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.15M sodium malate, 20% (w/v) PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
