2GJE
Structure of a guideRNA-binding protein complex bound to a gRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.989 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 157.600, 157.600, 81.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 69.850 - 3.370 |
R-factor | 0.2821 |
Rwork | 0.282 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.400 |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.600 | 3.450 |
High resolution limit [Å] | 3.370 | 3.370 |
Number of reflections | 8126 | |
Completeness [%] | 92.0 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | peg 4000, hepes, isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | peg 4000 | ||
2 | 1 | 1 | hepes | ||
3 | 1 | 1 | isopropanol | ||
4 | 1 | 2 | peg 4000 | ||
5 | 1 | 2 | hepes | ||
6 | 1 | 2 | isopropanol |