2GJA
Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and NH4+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Detector technology | CCD |
Collection date | 2005-04-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.950 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.280, 90.650, 138.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.370 - 1.850 |
R-factor | 0.19688 |
Rwork | 0.195 |
R-free | 0.22837 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.498 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.900 | |
High resolution limit [Å] | 1.850 | 6.000 | 1.850 |
Rmerge | 0.068 | 0.037 | 0.317 |
Number of reflections | 38156 | ||
<I/σ(I)> | 14.44 | 29.5 | 5.5 |
Completeness [%] | 97.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5 M ammonium sulphate, 100 mM HEPES 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |