2GJ3
Crystal structure of the FAD-containing PAS domain of the protein NifL from Azotobacter vinelandii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8266 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 68.111, 68.111, 302.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.040 |
| R-factor | 0.158 |
| Rwork | 0.154 |
| R-free | 0.18600 |
| Structure solution method | MAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 3.458 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.040 | 0.462 |
| Number of reflections | 126308 | 8336 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 94.5 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.25M Ammonium sulfate, 5% ethanol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
