2GIY

Crystal Structure of the C-terminal domain of the HSV-1 gE ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-24
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97011
Spacegroup name	P 1 21 1
Unit cell lengths	45.681, 91.242, 47.409
Unit cell angles	90.00, 117.64, 90.00

Refinement procedure

Resolution	36.470 - 1.780
Rwork	0.198
R-free	0.22000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.082	0.331
Number of reflections	32765	1203
<I/σ(I)>	16.1
Completeness [%]	98.6	72.6
Redundancy	5	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	28-32% (w/v) PEG 4000, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	28-32% (w/v) PEG 4000, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	28-32% (w/v) PEG 4000, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	28-32% (w/v) PEG 4000, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K