2GIS
Structure of the S-adenosylmethionine riboswitch mRNA regulatory element
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10532, 1.10573 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.901, 62.901, 158.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.900 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.426 |
| Number of reflections | 14940 | |
| <I/σ(I)> | 17.9 | 4.4 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 14.64 | 11.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
