2GII
Q138F HincII bound to cognate DNA GTTAAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.375, 90.040, 68.009 |
| Unit cell angles | 90.00, 103.45, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.140 |
| Rmerge | 0.051 |
| Number of reflections | 32583 |
| <I/σ(I)> | 24.8 |
| Completeness [%] | 99.9 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 25% PEG 4K, 0.15 M NaCl, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4K | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 4K | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | H2O |
