2GIB
Crystal structure of the SARS coronavirus nucleocapsid protein dimerization domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97940, 0.94285 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.163, 50.490, 41.479 |
| Unit cell angles | 90.00, 108.92, 90.00 |
Refinement procedure
| Resolution | 30.930 - 1.750 |
| R-factor | 0.18951 |
| Rwork | 0.187 |
| R-free | 0.23451 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.674 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.930 | 1.795 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 23088 | |
| Completeness [%] | 99.1 | 96.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.5 | 30-33% Pentaerythritol ethoxylate 15/4 EO/OH, 50 mM Ammonium sulfate, 50 mM Bis-Tris , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.5K |
