2GIA
Crystal structures of trypanosoma bruciei MRP1/MRP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.270, 85.700, 86.860 |
| Unit cell angles | 90.00, 109.38, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.890 |
| R-factor | 0.1791 |
| Rwork | 0.179 |
| R-free | 0.21600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 63446 | |
| Completeness [%] | 95.3 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | peg 4000, sodium acetate, ammonium acetate, glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
