2GI3
Crystal structure of Glutamyl-tRNA(Gln) amidotransferase subunit A (tm1272) from THERMOTOGA MARITIMA at 1.80 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.918381, 0.978662 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 131.776, 131.776, 61.987 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.910 - 1.800 |
R-factor | 0.17628 |
Rwork | 0.175 |
R-free | 0.20100 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.490 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.910 | 29.910 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.155 | 0.048 | 0.729 |
Number of reflections | 57320 | ||
<I/σ(I)> | 4.5 | 12.3 | 1 |
Completeness [%] | 99.8 | 98.3 | |
Redundancy | 6.9 | 6.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 277 | 0.02M CaCl2, 30.0% MPD, 0.1M Acetate pH 4.6 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |