2GHZ
Crystal structure of Azurin Phe114Pro mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.350, 47.810, 132.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.800 - 1.600 |
| R-factor | 0.14445 |
| Rwork | 0.142 |
| R-free | 0.19028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4AZU - chain A |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.047 | 0.204 |
| Number of reflections | 33091 | |
| <I/σ(I)> | 27.4 | 4.8 |
| Completeness [%] | 98.6 | 92.7 |
| Redundancy | 6.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 25% PEG1500, 100mM MMT buffer, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
