2GHY
Novel Crystal Form of the ColE1 Rom Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-01 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.770, 39.110, 26.700 |
Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.179 |
Rwork | 0.179 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gto |
RMSD bond length | 0.008 |
RMSD bond angle | 2.151 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 3487 | |
Completeness [%] | 97.5 | 96.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 30% PEG MME 2000, 0.1M sodium acetate, pH4.6, and 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |