2GHR

Crystal structure of homoserine o-succinyltransferase (NP_981826.1) from Bacillus cereus ATCC 10987 at 2.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918381, 0.979094,0.978532
Spacegroup name	P 41 2 2
Unit cell lengths	95.890, 95.890, 75.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.19144
Rwork	0.188
R-free	0.25000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.742
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.640	29.640	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.124	0.035	0.592
Number of reflections	14274
<I/σ(I)>	4.6	17.3	1.3
Completeness [%]	99.8
Redundancy	6.6	5.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	277	1.6M (NH4)2SO4, 0.1M TRIS pH 8.0 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K