2GHP

Crystal structure of the N-terminal 3 RNA binding domains of the yeast splicing factor Prp24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	114.608, 125.848, 196.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.503 - 2.700
Rwork	0.213
R-free	0.26420
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.380
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.503	49.500	2.800
High resolution limit [Å]	2.700	5.820	2.700
Rmerge	0.122	0.084	0.489
Number of reflections	74236
<I/σ(I)>	7.416		2.39
Completeness [%]	94.2	99.7	74.3
Redundancy	7	7.2	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.0003 M TCEP, 0.005 TRIS PH 8.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (14% MEPEG 5000, 0.20 M TETRAMETHYL AMMONIUM CHLORIDE, 0.10 M SODIUM SUCCINATE PH 4.0) Crystals cryo-protected with the well solution supplemented with 30% ethylene glycol., VAPOR DIFFUSION, HANGING DROP, temperature 277K