2GHI
Crystal Structure of Plasmodium yoelii Multidrug Resistance Protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.015, 70.689, 74.384 |
| Unit cell angles | 69.81, 76.69, 82.29 |
Refinement procedure
| Resolution | 32.670 - 2.200 |
| R-factor | 0.20568 |
| Rwork | 0.204 |
| R-free | 0.28834 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mv5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.881 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.670 |
| Number of reflections | 56948 | |
| <I/σ(I)> | 8 | 1.77 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 297 | 29%Peg 3350, 0.2M Li2SO4, 4% Glycerol, and 0.1M BisTris pH 5.5 , VAPOR DIFFUSION, HANGING DROP, temperature 297K |
