2GH7
Epi-biotin complex with core streptavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979454 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.390, 93.811, 104.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.141 |
| R-free | 0.15230 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.860 |
| High resolution limit [Å] | 0.850 | 0.850 |
| Rmerge | 0.045 | |
| Number of reflections | 189859 | |
| <I/σ(I)> | 55.5 | |
| Completeness [%] | 100.0 | 83 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | Protein solution - 10 mg/mL, 10mM Tris Hl, pH 7.0 Reservoir - 38% saturate ammonium sulfate, 0.1 M sodium acetate, pH 4.5,0.2 M NaCl , VAPOR DIFFUSION |
