2GH2
1.5 A Resolution R. Norvegicus Cysteine Dioxygenase Structure Crystallized in the Presence of Cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.484, 57.484, 122.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.840 - 1.500 |
| R-factor | 0.222 |
| Rwork | 0.194 |
| R-free | 0.22200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Native CDO (2B5H) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.840 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 33961 | |
| <I/σ(I)> | 25.1 | 1.4 |
| Completeness [%] | 100.0 | 87.5 |
| Redundancy | 13.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.15 M ammonium sulfate, 0.2 M sodium cacodylate, 26% PEG 8K, 5 mM cysteine , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
