2GH1
Crystal Structure of the putative SAM-dependent methyltransferase BC2162 from Bacillus cereus, Northeast Structural Genomics Target BcR20.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 |
| Unit cell lengths | 90.889, 90.889, 122.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.750 - 2.500 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.542 |
| Number of reflections | 38921 | |
| <I/σ(I)> | 12.29 | 2.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4 | 277 | 100 mM Sodium Citrate, 5.76M Potassium Acetate, and 5 mM DTT. , pH 4.0, Microbatch., temperature 277K |
