2GGL
The mutant A222C of Agrobacterium radiobacter N-carbamoyl-D-amino acid amidohydrolase
Replaces: 2FKUReplaces: 2BA4Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2003-04-13 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.379, 68.101, 138.191 |
Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.16975 |
Rwork | 0.168 |
R-free | 0.20832 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fo6 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.381 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 50489 | |
Completeness [%] | 94.8 | 93.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | lithium sulfate, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |