2GGJ
The mutant Y218C of Deinococcus Radiodurans N-acylamino acid racemase
Replaces: 2FKTReplaces: 2BAEExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 |
| Unit cell lengths | 116.514, 116.514, 120.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.16445 |
| Rwork | 0.162 |
| R-free | 0.20806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r0m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 55568 | |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | lithium sulfate, Tris-HCl, PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
