2GGD
CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.172, 44.719, 76.959 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gga |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.690 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.078 | 0.261 |
| Number of reflections | 43324 | |
| <I/σ(I)> | 24.3 | 6.8 |
| Completeness [%] | 96.1 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | (NH4)2SO4/KCL/PEG400/HEPES-NA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
