2GG8
Novel bacterial methionine aminopeptidase inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.701, 62.017, 53.255 |
| Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gg0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.923 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.043 | 0.243 |
| Number of reflections | 20901 | |
| <I/σ(I)> | 14.3 | 3.9 |
| Completeness [%] | 92.9 | 92.7 |
| Redundancy | 2.1 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7 | 298 | 10 mg/ml protein, 25% PEG 8000, 100 mM TRIS-HCl, 1-5 mM inhibitor, pH 7.0, batch, temperature 298K |
