2GFT
Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-02 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.273, 79.354, 103.906 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.18253 |
| Rwork | 0.180 |
| R-free | 0.23156 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.293 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.346 |
| Number of reflections | 31812 | |
| Completeness [%] | 88.0 | 81.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 24-35% (w/v) PEG 1500 Soak: few seconds, 0.3-0.5 M methyl- (1 4)-galactotetraoside, 15% PEG 1500 and 15% PEG 400., VAPOR DIFFUSION, HANGING DROP, temperature 296K |
