2GFP
Structure of the Multidrug Transporter EmrD from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.03840,1.04040 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.500, 124.500, 109.400 |
Unit cell angles | 90.00, 110.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.500 |
R-factor | 0.28 |
Rwork | 0.270 |
R-free | 0.35000 |
Structure solution method | MAD |
RMSD bond length | 0.050 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 13425 | |
Completeness [%] | 90.0 | 90 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 20-21% PEG 400, 50 mM sodium citrate, pH 4.4 and 4.8, and 50 mM KCl, VAPOR DIFFUSION, SITTING DROP |