2GFP
Structure of the Multidrug Transporter EmrD from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.03840,1.04040 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.500, 124.500, 109.400 |
| Unit cell angles | 90.00, 110.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.500 |
| R-factor | 0.28 |
| Rwork | 0.270 |
| R-free | 0.35000 |
| Structure solution method | MAD |
| RMSD bond length | 0.050 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 13425 | |
| Completeness [%] | 90.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 20-21% PEG 400, 50 mM sodium citrate, pH 4.4 and 4.8, and 50 mM KCl, VAPOR DIFFUSION, SITTING DROP |
