2GFI
Crystal structure of the phytase from D. castellii at 2.3 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.655, 121.655, 332.245 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.230 - 2.290 |
| Rwork | 0.154 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qfx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.512 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.541 | 2.420 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.110 | 0.219 |
| Total number of observations | 21949 | |
| Number of reflections | 62779 | 7925 |
| <I/σ(I)> | 5 | 3.1 |
| Completeness [%] | 95.2 | 83 |
| Redundancy | 5.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.02M CaCl2 15% MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
