2GFH

Crystal structure of a n-acetylneuraminic acid phosphatase (nanp) from mus musculus at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-24
Detector	ADSC QUANTUM 4
Spacegroup name	P 61 2 2
Unit cell lengths	71.259, 71.259, 195.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.820 - 1.900
R-factor	0.21
Rwork	0.208
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x42A
RMSD bond length	0.016
RMSD bond angle	1.513
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.059	0.045	0.470
Number of reflections	24076	1367	1120
<I/σ(I)>	15.6		3.34
Completeness [%]	99.2	96.7	93.8
Redundancy	8.9	9	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	9	277	1.8M (NH4)H2PO3, 0.1M TRIS, pH 9.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K