2GFC
cAMP-dependent protein kinase PKA catalytic subunit with PKI-5-24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Detector technology | CCD |
| Collection date | 2004-07-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00076 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.492, 75.533, 80.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.050 - 1.870 |
| R-factor | 0.19435 |
| Rwork | 0.192 |
| R-free | 0.23908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AN UNPUBLISHED STRUCTURE OF THE BINARY COMPLEX OF BOVINE PKA C ALPHA ENZYME WITH PKI(5-24) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.566 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.048 |
| High resolution limit [Å] | 1.642 |
| Number of reflections | 54188 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 15 % Methanol, 75 mM LiCl, 30 mM MesBisTris, 1 mM DTT, 0.2 mM EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
