2GF4
Crystal structure of Vng1086c from Halobacterium salinarium (Halobacterium halobium). Northeast Structural Genomics Target HsR14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97877 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.669, 64.914, 123.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.070 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.451 |
| Number of reflections | 29978 | 2992 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil | 5 | 293 | 0.55M Calcium acetate 0.1 M Sodium acetate; 1ul+1ul used 25% ethylene glycol as cryoprotectant (1.5ul mother liquor + 0.5ul 100% EG), pH 5.0, Under oil, temperature 293K |
