2GF2
Crystal structure of human hydroxyisobutyrate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97640 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.053, 122.745, 200.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.560 - 2.380 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.24677 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.140 | 0.526 |
Number of reflections | 50370 | |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.20M LiCl, 0.1M HEPES, 20.0% PEG 6K, 10.0% Ethylen Glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |