2GEX

Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	110
Detector technology	CCD
Collection date	2004-07-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790, 0.9788, 0.9823
Spacegroup name	P 32 2 1
Unit cell lengths	90.008, 90.008, 116.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.900 - 2.500
R-factor	0.21942
Rwork	0.218
R-free	0.24892
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.448
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	18909
<I/σ(I)>	26.1	2.4
Completeness [%]	98.0	87.8
Redundancy	13	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	18-20% PEG 8000, 0.2M magnesium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K