2GEX
Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-07-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9790, 0.9788, 0.9823 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 90.008, 90.008, 116.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.900 - 2.500 |
R-factor | 0.21942 |
Rwork | 0.218 |
R-free | 0.24892 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.448 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 18909 | |
<I/σ(I)> | 26.1 | 2.4 |
Completeness [%] | 98.0 | 87.8 |
Redundancy | 13 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18-20% PEG 8000, 0.2M magnesium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |