2GEW
Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.110, 73.256, 62.432 |
Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
Resolution | 31.300 - 0.970 |
R-factor | 0.113 |
Rwork | 0.113 |
R-free | 0.14000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1mxt |
RMSD bond length | 0.015 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.300 | 1.000 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.075 | 0.510 |
Number of reflections | 243484 | |
<I/σ(I)> | 11.8 | 1.9 |
Completeness [%] | 93.0 | 79 |
Redundancy | 4.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 8000, AMMONIUM SULFATE, PIPES PH 7.5,VAPOR DIFFUSION, HANGING DROP, temperature 298K, SOAK CONDITION STEP 1: PEG 8000, AMMONIUM SULFATE, HEPES PH 8.0 SOAK CONDITION STEP 2: PEG 8000, AMMONIUM SULFATE, TRICINE PH 8.5 SOAK CONDITION STEP 3: PEG 8000, AMMONIUM SULFATE, CHES PH 9.0, |