2GEN
Structural Genomics, the crystal structure of a probable transcriptional regulator from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 38.335, 69.130, 176.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.030 - 1.700 |
| R-factor | 0.21164 |
| Rwork | 0.210 |
| R-free | 0.24842 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.424 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.030 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 0.717 |
| Number of reflections | 26275 | |
| <I/σ(I)> | 43.6 | 2.45 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 9.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 1.2M di-Ammonium Tartrate, 0.1M NaAcetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
