2GEC
Structure of the N-terminal domain of avian infectious bronchitis virus nucleocapsid protein (strain Gray) in a novel dimeric arrangement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 178 |
| Detector technology | CCD |
| Collection date | 2003-12-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.055, 46.210, 74.176 |
| Unit cell angles | 90.00, 121.06, 90.00 |
Refinement procedure
| Resolution | 25.420 - 1.300 |
| R-factor | 0.20737 |
| Rwork | 0.205 |
| R-free | 0.24612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2BTL |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.230 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.056 | 0.227 |
| Number of reflections | 90638 | |
| <I/σ(I)> | 49.3 | 49.3 |
| Completeness [%] | 87.9 | 99.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 25 % PEG 4000,100 mM MES Sodium Salt, pH 6.2, 200 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
