2GE8
Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 178 |
Detector technology | CCD |
Collection date | 2003-12-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 108.990, 128.534, 71.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.780 - 2.200 |
R-factor | 0.24 |
Rwork | 0.237 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2GE7 4 copies |
RMSD bond length | 0.020 |
RMSD bond angle | 1.919 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.473 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.115 | 0.013 |
Total number of observations | 35383 | |
Number of reflections | 66258 | 9713 |
<I/σ(I)> | 4.8 | 0.5 |
Completeness [%] | 95.8 | |
Redundancy | 4.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 30% PEG 4000, 100 mM Tris-HCl pH 8.6, 800 mM LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |