2GE7
Structure of the C-terminal dimerization domain of infectious bronchitis virus nucleocapsid protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 178 |
Detector technology | CCD |
Collection date | 2004-06-24 |
Detector | SBC-2 |
Wavelength(s) | 0.97937 , 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.658, 65.707, 91.008 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.450 - 2.000 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.25400 |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 1.970 |
Data scaling software | SCALA |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.452 | 2.000 |
High resolution limit [Å] | 1.984 | 1.900 |
Rmerge | 0.072 | 0.282 |
Number of reflections | 15780 | |
<I/σ(I)> | 28.8 | 0.73 |
Completeness [%] | 96.0 | 95 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | PEG 4000, 28.75% to 29.5 %, pH 4.8 Citrate, 0.1 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |