2GE5
EcoRV Restriction Endonuclease C-terminal deletion mutant/GATATC/Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 297 |
| Collection date | 2005-04-09 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.101, 67.101, 259.892 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.223 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eop |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.230 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.677 | 46.680 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.067 | 0.042 | 0.467 |
| Total number of observations | 4550 | 2163 | |
| Number of reflections | 16007 | 778 | 694 |
| <I/σ(I)> | 8.6 | 14.6 | 1.6 |
| Completeness [%] | 65.9 | 45.1 | |
| Redundancy | 6 | 5.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 250 mM NaCl, 8-12% PEG 4k, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCl | ||
| 2 | 1 | 1 | PEG 4k | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | NaCl | ||
| 5 | 1 | 2 | PEG 4k | ||
| 6 | 1 | 2 | H2O |
