2GCY
humanized antibody C25 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115870 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.260, 90.260, 217.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.600 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.600 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 17714 |
| Completeness [%] | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG MME 550, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
