2GCP
Crystal structure of the human RhoC-GSP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.435, 59.435, 138.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.550 - 2.150 |
| R-factor | 0.16229 |
| Rwork | 0.159 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.733 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.593 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.122 | 0.583 |
| Total number of observations | 24100 | |
| Number of reflections | 14205 | 2019 |
| <I/σ(I)> | 6.1 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291.15 | 3M Sodium Chloride, 0.1M Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
